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gga3 pbd beads  (Cytoskeleton Inc)


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  • 90

    Structured Review

    Cytoskeleton Inc gga3 pbd beads
    Gga3 Pbd Beads, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gga3+pbd+beads/GGA3-PBD+Arf+Affinity+Beads/pmc08790574-61-10-12
    Average 90 stars, based on 4 article reviews
    gga3 pbd beads - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: GEF Cytohesin-2/ARNO: a novel bridge between cell migration and immunoregulation in synovial fibroblasts
    Article Snippet: .. Then, cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein. .. The beads were then resuspended and boiled in Laemmli buffer (SigmaAldrich, UK) and the samples resolved by SDS-PAGE prior to detection of ARF6 by Western blot.

    Article Title: Cytohesin-2/ARNO: A Novel Bridge Between Cell Migration and Immunoregulation in Synovial Fibroblasts
    Article Snippet: .. Then, cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein. .. The beads were then resuspended and boiled in Laemmli buffer (SigmaAldrich, UK) and the samples resolved by SDS-PAGE prior to detection of ARF6 by Western blot.

    Article Title: GGA3-mediated recycling of the RET receptor tyrosine kinase contributes to cell migration and invasion.
    Article Snippet: The RET receptor tyrosine kinase plays important roles in regulating cellular proliferation, migration, and survival in the normal development of neural crest derived tissues.. However, aberrant activation of RET, through oncogenic mutations or overexpression, can contribute to tumourigenesis, regional invasion, and metastasis of several human cancers.. RET is expressed as two main isoforms with unique C-terminal sequences that differ in protein interactions and subcellular trafficking in response to RET activation, and which also have distinct oncogenic potentials.

    Activity Assay:

    Article Title: GGA3-mediated recycling of the RET receptor tyrosine kinase contributes to cell migration and invasion.
    Article Snippet: The RET receptor tyrosine kinase plays important roles in regulating cellular proliferation, migration, and survival in the normal development of neural crest derived tissues.. However, aberrant activation of RET, through oncogenic mutations or overexpression, can contribute to tumourigenesis, regional invasion, and metastasis of several human cancers.. RET is expressed as two main isoforms with unique C-terminal sequences that differ in protein interactions and subcellular trafficking in response to RET activation, and which also have distinct oncogenic potentials.



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